Observations of uridine diphosphate glucuronyltransferase activity towards oestriol and xenobiotics in developing and cultured tissues from mouse and man.
نویسنده
چکیده
Non-diffusible residues in the medium were obtained from stationary-phasespent culture medium from Pseudomoms no. 95 by thorough dialysis and freeze-drying. These were added to other cultures at the start of growth and the distribution of 14C between EDTAextractable lipopolysaccharides, non-EDTA-extractable lipopolysaccharides, the remainder of the bacterium and the culture fluid was determined.
منابع مشابه
Postnatal development of uridine diphosphate glucuronyltransferase activity towards bilirubin and 2-aminophenol in human liver.
UDP-glucuronyltransferase activities towards 2-aminophenol and bilirubin were studied in a total of 70 human subjects, including premature and full-term newborn babies, infants, children and adults. These two activities have been reported in rat to develop latefoetally and neonatally respectively, but in man they both develop neonatally. There is a linear relationship between the logarithm of e...
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An enzyme that conjugates the 17beta-hydroxyl group of testosterone was found in the cytosol fraction of human liver. The same enzyme preparation also conjugates the 16alpha-hydroxyl group of oestriol. The enzymic activity could not be sedimented by centrifuging the cytosol fraction at 158000g(av.) for 120min. The testosterone-conjugating as well as the oestriol-conjugating activities were foun...
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1. A stable, more highly purified, preparation of UDP-glucuronyltransferase was obtained than previously reported. 2. Enzyme activity towards o-aminophenyl and p-nitrophenyl was increased 43- and 46-fold respectively. 3. The final preparation contains only three staining polypeptide bands visible after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 4. The only known major accompany...
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The glucuronic acid adducts of 1-naphthol, 2-naphthol and 4-methylumbelliferone activate microsomal UDP-glucuronyltransferase (EC 2.4.1.17) when the enzyme is assayed with p-nitrophenol as aglycone. Phenyl glucuronide and oestriol 3beta-glucuronide also activate UDP-glucuronyltransferase. but to a lesser extent. Activation by glucuronides is not dependent on metal ions, but is blocked by prior ...
متن کاملThe biosynthesis and induction of microsomal UDP-glucuronyltransferase in avian liver.
Introduction The molecular mechanisms involved in the regulation of hepatic microsomal UDP-glucuronyltransferases are currently the subject of rigorous investigations by a number of laboratories. Overwhelming evidence indicates that UDPglucuronyltransferase is a heterogeneous family of enzymes (see Burchell, 1981); thus these investigations in mammalian tissues are complicated by the specificit...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 1 5 شماره
صفحات -
تاریخ انتشار 1973